6MJC

Structure of Candida glabrata Csm1:Dsn1(43-67DD) complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-22
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	H 3 2
Unit cell lengths	105.057, 105.057, 125.121
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	52.529 - 1.790
R-factor	0.2001
Rwork	0.199
R-free	0.22320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3n4r
RMSD bond length	0.012
RMSD bond angle	1.180
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.000	1.830
High resolution limit [Å]	1.790	1.790
Rmerge	0.030	1.440
Rmeas	0.034	1.025
Rpim	0.016	0.753
Number of reflections	25112	1462
<I/σ(I)>	20
Completeness [%]	99.7
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	0.1 M sodium acetate, pH 4.5, 3 M sodium chloride, cryoprotectant: 0.5 M sodium chloride, 0.5 M sodium malonate, pH 4.5