6MJ0

Crystal structure of the complete turnip yellow mosaic virus 3'UTR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2017-05-16
Detector	RDI CMOS_8M
Wavelength(s)	1.072
Spacegroup name	I 2 2 2
Unit cell lengths	103.032, 129.126, 172.005
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.790 - 3.100
R-factor	0.2303
Rwork	0.227
R-free	0.26620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4p5j
RMSD bond length	0.008
RMSD bond angle	1.517
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((1.12_2829))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.790	3.200
High resolution limit [Å]	3.100	3.100
Rmerge	0.088	1.690
Rmeas	0.095	1.830
Rpim	0.036	0.690
Number of reflections	21141	1352
<I/σ(I)>	16.69	1.37
Completeness [%]	89.4	64.37
Redundancy	7.1	6.9
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293.15	5 mg/mL RNA was resuspended in 2.5 mM magnesium chloride and 10 mM HEPES KOH pH 7.4 and DPEC treated water. The RNA was heated at 65 degrees Celsius and slow cooled to room temperature followed by the addition of spermidine (0.5 mM final). Crystals were grown in 50 mM cacodylate, 2.5 M ammonium sulfate, 20 mM magnesium chloride, 1.0 mM spermine, and 2 mM hexamine cobalt chloride