6MIT

LptBFGC from Enterobacter cloacae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	80
Detector technology	PIXEL
Collection date	2017-11-29
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.03324
Spacegroup name	P 21 2 21
Unit cell lengths	116.427, 156.984, 295.518
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.326 - 3.200
R-factor	0.2798
Rwork	0.279
R-free	0.32010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5l75
Data reduction software	XDS
Data scaling software	Aimless (0.7.1)
Phasing software	PHASER (2.8.1)
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.330	49.330	3.050
High resolution limit [Å]	3.000	16.430	3.000
Rmerge	0.480	0.095
Rmeas	0.499	0.098	86.592
Rpim	0.130	0.024
Total number of observations	1745941	11806	64991
Number of reflections	109124	744	5337
<I/σ(I)>	5.6	20	0.2
Completeness [%]	100.0	96.1	99.7
Redundancy	16	15.9	12.2
CC(1/2)	0.723	0.998	0.137

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	21% PEG 400, 100mM MES pH 6.5, 500mM Li2SO4, 2mM Na-novobiocin