6MIN
Crystal structure of Taf14 YEATS domain G82A mutant in complex with histone H3K9cr
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-30 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 65 |
| Unit cell lengths | 113.446, 113.446, 26.392 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.134 - 1.900 |
| R-factor | 0.207 |
| Rwork | 0.202 |
| R-free | 0.25560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5iok |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.872 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.140 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 15267 | |
| <I/σ(I)> | 40 | |
| Completeness [%] | 96.7 | |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 44% PEG600 (v/v) and 0.2 M citric acid (pH 6.0) |
