6MI6

STRUCTURE OF CHEA DOMAIN P4 IN COMPLEX WITH AN ADP ANALOG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97220
Spacegroup name	P 1 21 1
Unit cell lengths	83.703, 75.145, 86.580
Unit cell angles	90.00, 90.01, 90.00

Refinement procedure

Resolution	10.000 - 2.950
R-factor	0.2398
Rwork	0.233
R-free	0.29770
RMSD bond length	0.015
RMSD bond angle	1.771
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.053
High resolution limit [Å]	2.950	2.950
Rmerge	0.059
Rmeas	0.071
Rpim	0.040
Number of reflections	25447	1692
<I/σ(I)>	5.7
Completeness [%]	93.4	99.27
Redundancy	3.2	2
CC(1/2)	0.459

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	290	50 mM Citrate pH 5.5, 2 M Ammonium Sulfate