6MHI
Photoactive Yellow Protein with covalently bound 3,5-dichloro-4-hydroxycinnamic acid chromophore
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2017-08-23 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.88558 |
| Spacegroup name | P 63 |
| Unit cell lengths | 65.970, 65.970, 40.420 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.460 - 1.350 |
| Rwork | 0.143 |
| R-free | 0.18630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nwz |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.460 | |
| High resolution limit [Å] | 1.280 | 1.280 |
| Rmerge | 0.052 | |
| Rpim | 0.013 | |
| Number of reflections | 24905 | |
| <I/σ(I)> | 25.1 | |
| Completeness [%] | 62.8 | |
| Redundancy | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 1M sodium chloride, 2.4M ammonium sulfate, pH = 6.0, drops were microseeded |
