6MH8
High-viscosity injector-based Pink Beam Serial Crystallography of Micro-crystals at a Synchrotron Radiation Source
Experimental procedure
| Experimental method | LAUE |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2017-06-24 |
| Detector | RAYONIX MX340-HS |
| Wavelength(s) | 1.033-1.67 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 40.000, 179.000, 142.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 4.200 |
| R-factor | 0.25385 |
| Rwork | 0.250 |
| R-free | 0.28767 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5uvi |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.043 |
| Data reduction software | CrystFEL |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.300 |
| High resolution limit [Å] | 4.200 | 4.200 |
| Number of reflections | 4021 | |
| <I/σ(I)> | 4.5 | |
| Completeness [%] | 87.8 | |
| Redundancy | 11.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 5 | 298 | sodium citrate pH 5.0, PEG 400, sodium thiocyanate |
