6MH6
High-viscosity injector-based Pink Beam Serial Crystallography of Micro-crystals at a Synchrotron Radiation Source.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-D |
| Synchrotron site | APS |
| Beamline | 14-BM-D |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2016-08-20 |
| Detector | RAYONIX MX340-HS |
| Wavelength(s) | 1.03 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 68.300, 68.300, 108.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.800 - 1.800 |
| R-factor | 0.13408 |
| Rwork | 0.130 |
| R-free | 0.17493 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5uvl |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.802 |
| Data reduction software | Precognition |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.800 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.102 | |
| Number of reflections | 14910 | |
| <I/σ(I)> | 23.3 | |
| Completeness [%] | 61.3 | |
| Redundancy | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 6.5 | 293 | 0.1 M MES pH 6.5, 0.5 M sodium nitrate, 0.1 M calcium chloride |
