6MGK
Crystal structure of the catalytic domain from GH74 enzyme PoGH74 from Paenibacillus odorifer, in complex with XLX xyloglucan
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-26 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.978 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 239.263, 212.471, 91.647 |
| Unit cell angles | 90.00, 105.50, 90.00 |
Refinement procedure
| Resolution | 39.290 - 2.100 |
| R-factor | 0.159 |
| Rwork | 0.158 |
| R-free | 0.18100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cn3 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.693 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((DEV_3092: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.093 | 0.479 |
| Number of reflections | 252675 | 252675 |
| <I/σ(I)> | 11.4 | 2.21 |
| Completeness [%] | 99.0 | 97.1 |
| Redundancy | 3.6 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 0.2 M potassium sodium tartrate, 20% PEG3350, 5 mM xyloglucan mixture |
