6MGE

Structure of human 4-1BBL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-20
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9774
Spacegroup name	P 62
Unit cell lengths	118.910, 118.910, 105.494
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.947 - 2.950
R-factor	0.1971
Rwork	0.194
R-free	0.24650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2x29
RMSD bond length	0.003
RMSD bond angle	0.612
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.950	3.056
High resolution limit [Å]	2.950	2.950
Number of reflections	17937	1796
<I/σ(I)>	13.25	0.7
Completeness [%]	99.8	99.05
Redundancy	10.3	10.2
CC(1/2)	0.997	0.340

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	8% w/v PEG 8000, 100mM Tris 8.5