6MG3
V285A Mutant of the C-terminal bZIP domain of human C/EBPbeta with 16bp Methylated Oligonucleotide Containing Consensus Recognition Sequence
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | RIGAKU MICROMAX-003 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-26 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54218 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 101.430, 114.042, 75.444 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.734 - 2.050 |
| R-factor | 0.2229 |
| Rwork | 0.222 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2e42 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.651 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.734 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.172 | |
| Rpim | 0.064 | 0.677 |
| Number of reflections | 28807 | 2741 |
| <I/σ(I)> | 9.5 | 1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.2 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 292 | 0.1 M sodium acetate trihydrate pH 7.0, 12% (w/v) polyethylene glycol 3350 |
