6MFZ

Crystal structure of dimodular LgrA in a condensation state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-15
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979
Spacegroup name	C 2 2 21
Unit cell lengths	213.601, 262.746, 249.096
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	78.633 - 6.000
R-factor	0.2565
Rwork	0.255
R-free	0.27870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6mfw
RMSD bond length	0.004
RMSD bond angle	0.698
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((dev_3494: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	78.633	6.710
High resolution limit [Å]	6.000	6.000
Number of reflections	17913	5026
<I/σ(I)>	6.9	2.1
Completeness [%]	100.0
Redundancy	12.8
CC(1/2)		0.411

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	0.25 M sodium/potassium phosphate, 21% PEG3350, 0.1 M Bis-Tris propane, pH 7.5