6MFZ
Crystal structure of dimodular LgrA in a condensation state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-15 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 213.601, 262.746, 249.096 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 78.633 - 6.000 |
| R-factor | 0.2565 |
| Rwork | 0.255 |
| R-free | 0.27870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6mfw |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.698 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_3494: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.633 | 6.710 |
| High resolution limit [Å] | 6.000 | 6.000 |
| Number of reflections | 17913 | 5026 |
| <I/σ(I)> | 6.9 | 2.1 |
| Completeness [%] | 100.0 | |
| Redundancy | 12.8 | |
| CC(1/2) | 0.411 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.25 M sodium/potassium phosphate, 21% PEG3350, 0.1 M Bis-Tris propane, pH 7.5 |
