6MFV

Crystal structure of the Signal Transduction ATPase with Numerous Domains (STAND) protein with a tetratricopeptide repeat sensor PH0952 from Pyrococcus horikoshii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-03-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9801
Spacegroup name	P 65
Unit cell lengths	96.126, 96.126, 584.259
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.688 - 3.400
R-factor	0.2263
Rwork	0.224
R-free	0.27160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Obtained by SAD phasing
RMSD bond length	0.002
RMSD bond angle	0.482
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.690	3.540
High resolution limit [Å]	3.400	3.400
Rmerge	0.093	0.791
Rpim	0.042	0.355
Number of reflections	41809	27458
<I/σ(I)>	8.9
Completeness [%]	100.0
Redundancy	5.8
CC(1/2)	0.999	0.884

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	10% (v/v) 2-propanol, 0.1 M imidazole pH 8