6MFO
Crystal Structure of Human Protocadherin-15 EC1-3 G16D N369D Q370N
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-07 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97920 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 87.894, 116.516, 99.889 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.990 - 3.150 |
| R-factor | 0.21806 |
| Rwork | 0.215 |
| R-free | 0.28841 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4apx 5uly |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.361 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.200 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 0.362 | 1.384 |
| Rpim | 0.119 | 0.616 |
| Number of reflections | 9312 | 460 |
| <I/σ(I)> | 6.8 | |
| Completeness [%] | 99.4 | 99.8 |
| Redundancy | 9 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.7 | 277 | 0.1 M HEPES pH 7.7, 66% MPD, 4% Glycerol |
