6MFI
MIM-2 Metallo-Beta-Lactamase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2016-07-13 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 0.7107 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 51.672, 69.527, 76.541 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.826 - 1.839 |
| R-factor | 0.2641 |
| Rwork | 0.260 |
| R-free | 0.30900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sml |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.568 |
| Data reduction software | HKL-2000 |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.830 | 1.905 |
| High resolution limit [Å] | 1.839 | 1.839 |
| Rmerge | 0.168 | |
| Rpim | 0.060 | |
| Number of reflections | 24468 | 24436 |
| <I/σ(I)> | 7.5 | |
| Completeness [%] | 99.3 | 99.23 |
| Redundancy | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 323 | 0.1 mM DS56E8 (a detergent), 0.1 M sodium citrate, pH 5.5, and 22% w/v PEG-3350 |
