6MEZ
X-ray structure of the Fenna-Matthews-Olsen antenna complex from Prosthecochloris aestuarii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-12-12 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5417 |
| Spacegroup name | H 3 |
| Unit cell lengths | 83.638, 83.638, 294.781 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.650 - 1.740 |
| R-factor | 0.1336 |
| Rwork | 0.132 |
| R-free | 0.15860 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.297 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.650 | 1.804 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmerge | 0.061 | 0.409 |
| Number of reflections | 73467 | 3850 |
| <I/σ(I)> | 2.4 | 2.4 |
| Completeness [%] | 93.3 | 49.16 |
| Redundancy | 3.6 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 8 | 293 | 25 % Ammonium Sulfate and 0.1 M Sodium acetate pH 5.0 |
