Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-05-22 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.230, 29.870, 97.890 |
| Unit cell angles | 90.00, 102.12, 90.00 |
Refinement procedure
| Resolution | 34.700 - 1.780 |
| R-factor | 0.1782 |
| Rwork | 0.176 |
| R-free | 0.21870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | selected coordinates from pdb entry 3uxg |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.853 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.2) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 34.710 | 34.710 | 1.810 |
| High resolution limit [Å] | 1.780 | 9.050 | 1.780 |
| Rmerge | 0.062 | 0.030 | 0.499 |
| Rmeas | 0.067 | 0.033 | 0.541 |
| Rpim | 0.025 | 0.013 | 0.206 |
| Total number of observations | 245359 | 1917 | 12001 |
| Number of reflections | 35385 | ||
| <I/σ(I)> | 19.5 | 38.1 | 3.4 |
| Completeness [%] | 98.7 | 98.6 | 94.1 |
| Redundancy | 6.9 | 6.1 | 6.5 |
| CC(1/2) | 0.999 | 0.999 | 0.876 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 25% PEG-3350, 0.2 M ammonium acetate, 0.1 M bis-tris |
