6MER
PcdhgB3 EC1-4 in 50 mM HEPES
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2016-06-21 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.07 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 128.390, 161.770, 52.160 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.834 - 3.000 |
| R-factor | 0.2291 |
| Rwork | 0.224 |
| R-free | 0.27210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5k8r |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.390 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.13rc1_2961)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.834 | 3.107 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.144 | |
| Rmeas | 0.167 | |
| Number of reflections | 10828 | |
| <I/σ(I)> | 9.36 | |
| Completeness [%] | 95.8 | |
| Redundancy | 3.6 | |
| CC(1/2) | 0.988 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 100 mM Tris pH 7, 10% PEG 5000MME, 4% ethylene glycol |
