6MEL
Succinyl-CoA synthase from Campylobacter jejuni
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-28 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 123.072, 123.072, 146.788 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.900 - 2.060 |
| R-factor | 0.151 |
| Rwork | 0.150 |
| R-free | 0.17530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jkj |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.517 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.100 |
| High resolution limit [Å] | 2.060 | 5.590 | 2.060 |
| Rmerge | 0.104 | 0.048 | 0.949 |
| Rmeas | 0.110 | 0.051 | 1.014 |
| Rpim | 0.035 | 0.017 | 0.352 |
| Number of reflections | 80196 | 4255 | 3960 |
| <I/σ(I)> | 6.7 | 1.8 | |
| Completeness [%] | 100.0 | 99.8 | 99.9 |
| Redundancy | 9.8 | 9.4 | 8.1 |
| CC(1/2) | 0.999 | 0.670 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 1.0 M sodium citrate, 0.1 M cacodylate buffer |
