6MD5
Crystal structure of zebrafish Vps26A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-01 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.850, 75.480, 100.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.400 - 1.700 |
| R-factor | 0.1841 |
| Rwork | 0.182 |
| R-free | 0.21910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fau |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.122 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.400 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 34105 | 1808 |
| <I/σ(I)> | 16 | 2.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.1 | 14.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.2 M Ammonium sulfate, 0.1 M Bis-tris pH 5.5, 25 %(w/v) PEG 3350 |
