6MCV

Crystal Structure of Holo Retinal-Bound Domain-Swapped Dimer of Wild Type Human Cellular Retinol Binding Protein II

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2016-11-26
Detector	RAYONIX MX300HE
Wavelength(s)	0.97872
Spacegroup name	P 21 21 21
Unit cell lengths	65.819, 74.362, 352.547
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.993 - 3.300
R-factor	0.2098
Rwork	0.208
R-free	0.26580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2rct
RMSD bond length	0.001
RMSD bond angle	0.348
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	14.993	3.360
High resolution limit [Å]	3.300	3.300
Rmerge	0.190
Rmeas	0.200
Rpim		0.303
Number of reflections	24088	2395
<I/σ(I)>	17.1
Completeness [%]	89.8	92.12
Redundancy	10.5
CC(1/2)		0.773

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		298	4000 PEG, Ammonium acetate, Sodium acetate