6MBI

Crystal Structure of SNX15 PX domain in domain swapped conformation form 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9796
Spacegroup name	P 43 21 2
Unit cell lengths	60.312, 60.312, 85.161
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.219 - 2.834
R-factor	0.282
Rwork	0.278
R-free	0.31910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ecm
RMSD bond length	0.002
RMSD bond angle	0.517
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.220	2.980
High resolution limit [Å]	2.830	2.830
Rmerge	0.094	0.728
Rpim	0.028	0.210
Number of reflections	4100	565
<I/σ(I)>	14.4	2.6
Completeness [%]	99.4	96.2
Redundancy	12.2	12.6
CC(1/2)	0.999	0.960

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	4 M NaCl, 0.1 Tris (pH 8.5)