6MAS

X-ray Structure of Branchiostoma floridae fluorescent protein lanFP10G

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2018-01-01
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	77.875, 104.309, 116.142
Unit cell angles	90.00, 95.32, 90.00

Refinement procedure

Resolution	29.820 - 1.300
R-factor	0.1198
Rwork	0.119
R-free	0.14670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hvf
RMSD bond length	0.011
RMSD bond angle	1.765
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.350
High resolution limit [Å]	1.300	2.800	1.300
Rmerge	0.059	0.027	0.556
Rmeas	0.069	0.032	0.653
Rpim	0.035	0.016	0.338
Total number of observations	1703469
Number of reflections	451144	45608	44978
<I/σ(I)>	8.5
Completeness [%]	100.0	100	100
Redundancy	3.8	3.9	3.7
CC(1/2)		0.998	0.764

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.1M Imidazole pH 7.0, 20% PEG 6,000