Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2017-11-03 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 61.400, 61.400, 155.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.941 - 2.600 |
| R-factor | 0.2549 |
| Rwork | 0.252 |
| R-free | 0.30690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ngo |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.678 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.690 |
| Rpim | 0.063 | 0.329 |
| Number of reflections | 9820 | 454 |
| <I/σ(I)> | 10.53 | 2.05 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 5.7 | 3.8 |
| CC(1/2) | 0.954 | 0.814 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | Magnesium Chloride, HEPES pH 7.5, PEG 400 |
