6MAF
native BbvCI A2B2 tetramer at low resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | Cu FINE FOCUS |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-06-01 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 |
| Unit cell lengths | 113.152, 113.152, 115.001 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.400 - 3.790 |
| R-factor | 0.30167 |
| Rwork | 0.297 |
| R-free | 0.38792 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6eg7 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.653 |
| Data reduction software | HKL-2000 (V7.2) |
| Data scaling software | HKL-2000 (V7.2) |
| Phasing software | PHASER (V2.6.1) |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.400 | 3.889 |
| High resolution limit [Å] | 3.790 | 3.792 |
| Rmeas | 0.104 | |
| Rpim | 0.104 | |
| Number of reflections | 15368 | 1117 |
| <I/σ(I)> | 11.4 | |
| Completeness [%] | 98.9 | 93.93 |
| Redundancy | 5.1 | 5 |
| CC(1/2) | 0.784 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 15% PEF4k, 0.2M Ammonium Sulfate, Citrate pH 5.6 |
