6M98
Crystal structure of the high-affinity copper transporter Ctr1 in complex with Cu(I)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-15 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.3776 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 73.540, 73.540, 409.820 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 3.210 |
| R-factor | 0.30448 |
| Rwork | 0.301 |
| R-free | 0.33973 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.152 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.083 | 1.178 |
| Rpim | 0.035 | 0.501 |
| Number of reflections | 7470 | 713 |
| <I/σ(I)> | 21.7 | 1.2 |
| Completeness [%] | 99.6 | |
| Redundancy | 6.9 | 6 |
| CC(1/2) | 0.779 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 50 mM zinc acetate, 50 mM sodium cacodylate pH 5.9, and 28% PEG 400. |
