6M92

Monophosphorylated pSer33 b-Catenin peptide, b-TrCP/Skp1, NRX-2663 ternary complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-09-14
Detector	RAYONIX MX-300
Wavelength(s)	0.9795
Spacegroup name	P 31
Unit cell lengths	82.590, 82.590, 111.110
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.295 - 2.350
R-factor	0.19
Rwork	0.189
R-free	0.22080
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	41.295	41.295	2.410
High resolution limit [Å]	2.350	10.510	2.350
Rmerge	0.046	0.019	1.526
Rmeas	0.052	0.022	1.725
Number of reflections	35242	347	2583
<I/σ(I)>	18.29	54.77	0.92
Completeness [%]	99.9	88.5	100
Redundancy	4.838	4.435	4.544
CC(1/2)	0.999	0.999	0.382

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	8% PEG 4000 0.1M BTP pH 5.5