6M8T
Crystal structure of UbiX-like FMN prenyltransferase AF1214 from Archaeoglobus fulgidus, FMN complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-08-13 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 98.560, 98.560, 98.560 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.237 - 1.670 |
| R-factor | 0.1494 |
| Rwork | 0.148 |
| R-free | 0.17360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ejb |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.020 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((dev_3092: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.240 | 1.710 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Rmerge | 0.061 | 1.380 |
| Rpim | 0.024 | 0.550 |
| Number of reflections | 18632 | 1371 |
| <I/σ(I)> | 22.4 | 1.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.4 | 7.2 |
| CC(1/2) | 1.000 | 0.563 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 0.05 M Tris pH 8.5, 5 mM magnesium chloride, 50 mM potassium chloride, 5 mM ammonium sulfate, 30% (w/v) PEG400 cryoprotectant 5% PEG200 |
