6M8R
Crystal structure of the KCTD16 BTB domain in complex with GABAB2 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2017-06-07 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 91.964, 64.945, 114.137 |
| Unit cell angles | 90.00, 99.79, 90.00 |
Refinement procedure
| Resolution | 38.639 - 3.200 |
| R-factor | 0.2208 |
| Rwork | 0.217 |
| R-free | 0.26540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5a15 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.637 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3211: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.260 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.332 | 0.997 |
| Rmeas | 0.394 | 1.226 |
| Rpim | 0.208 | 0.703 |
| Number of reflections | 22162 | 1073 |
| <I/σ(I)> | 3.4 | 0.6 |
| Completeness [%] | 99.2 | 96.7 |
| Redundancy | 3.3 | 2.7 |
| CC(1/2) | 0.926 | 0.561 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM magnesium chloride, 100 mM Tris-HCl, pH 7.5, 12% w/v PEG8000 |
