6M8N

Endo-fucoidan hydrolase P5AFcnA from glycoside hydrolase family 107

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-03
Detector	DECTRIS PILATUS3 R 200K-A
Wavelength(s)	1.5418
Spacegroup name	P 31 2 1
Unit cell lengths	97.455, 97.455, 96.684
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.710 - 1.550
R-factor	0.1601
Rwork	0.159
R-free	0.18050
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.785
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0230)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	1.580
High resolution limit [Å]	1.550	4.210	1.550
Rmerge	0.068	0.062	0.565
Rmeas	0.071	0.064	0.629
Rpim	0.022	0.017	0.273
Number of reflections	77250	4096	3832
<I/σ(I)>	13.2
Completeness [%]	100.0	100	100
Redundancy	7.8	14.5	5.2
CC(1/2)		0.998	0.837

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	Sodium malonate pH 6-7