6M85

Crystal Structure of Inward Rectifier Kir2.2 in a different salt condition

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2017-02-11
Detector	ADSC QUANTUM 315
Wavelength(s)	0.98
Spacegroup name	I 4
Unit cell lengths	82.989, 82.989, 191.794
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.710
R-factor	0.2291
Rwork	0.228
R-free	0.25360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3spc
RMSD bond length	0.005
RMSD bond angle	0.906
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.800
High resolution limit [Å]	2.700	5.810	2.700
Rmerge	0.047	0.035	0.866
Rmeas	0.052	0.039	0.951
Rpim	0.020	0.015	0.389
Total number of observations	114715
Number of reflections	17592	1782	1751
<I/σ(I)>	11.7
Completeness [%]	99.9	99.4	99.7
Redundancy	6.5	6.6	5.7
CC(1/2)		0.997	0.833

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		298	PEG400, Trisodium Citrate, Tris