6M7L
Complex of OxyA with the X-domain from GPA biosynthesis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-11-27 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.953700 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 118.834, 87.344, 95.955 |
| Unit cell angles | 90.00, 95.77, 90.00 |
Refinement procedure
| Resolution | 47.735 - 2.648 |
| R-factor | 0.216600287234 |
| Rwork | 0.214 |
| R-free | 0.26415 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4tx3 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.541 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX (1.13-2998) |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.140 | 2.780 |
| High resolution limit [Å] | 2.640 | 2.650 |
| Rmerge | 0.037 | 0.500 |
| Rmeas | 0.052 | |
| Rpim | 0.035 | 0.464 |
| Number of reflections | 28454 | 3722 |
| <I/σ(I)> | 21.3 | 2.1 |
| Completeness [%] | 99.7 | 98.9 |
| Redundancy | 3.8 | 3.8 |
| CC(1/2) | 0.999 | 0.837 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298.15 | 30% PEG3350 300 mM NaCl 100 mM BIS-Tris |
