6M7G
Crystal structure of ArsN, N-acetyltransferase with substrate phosphinothricin from Pseudomonas putida KT2440
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-04-03 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.841, 142.693, 178.308 |
| Unit cell angles | 90.00, 89.99, 90.00 |
Refinement procedure
| Resolution | 39.908 - 2.657 |
| R-factor | 0.2267 |
| Rwork | 0.224 |
| R-free | 0.27260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5jtf |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.880 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.908 | 2.700 |
| High resolution limit [Å] | 2.657 | 2.657 |
| Rmeas | 0.178 | 1.320 |
| Number of reflections | 75010 | 3762 |
| <I/σ(I)> | 11 | |
| Completeness [%] | 97.0 | 97.2 |
| Redundancy | 4.2 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 0.1 M Sodium acetate, 1.5 M Sodium formate |
