6M7F
Wild-type Cucumene Synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 300 |
| Detector technology | PIXEL |
| Collection date | 2017-03-01 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.741, 95.846, 102.299 |
| Unit cell angles | 90.00, 96.92, 90.00 |
Refinement procedure
| Resolution | 29.897 - 3.051 |
| R-factor | 0.2472 |
| Rwork | 0.244 |
| R-free | 0.29930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ps1 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.573 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.900 | 3.160 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Rmerge | 0.147 | 0.672 |
| Rpim | 0.093 | 0.424 |
| Number of reflections | 23205 | 23205 |
| <I/σ(I)> | 5.7 | |
| Completeness [%] | 98.6 | 92.6 |
| Redundancy | 3.4 | 3.4 |
| CC(1/2) | 0.995 | 0.876 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 25% PEG 3350, 0.1mM Bis-Tris |
