6M7B

Structure of REV7-R124A complexed with SHLD3(37-73)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-14
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9785
Spacegroup name	P 21 21 21
Unit cell lengths	57.125, 62.286, 131.686
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.770
Rwork	0.175
R-free	0.22350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6m7a
RMSD bond length	0.007
RMSD bond angle	0.855
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.16-3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.830
High resolution limit [Å]	1.330	1.770
Rmerge	0.111	0.936
Number of reflections	46748	4624
<I/σ(I)>	2
Completeness [%]	100.0
Redundancy	11.5
CC(1/2)		0.589

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M NaCl Sodium Chloride; 0.1M HEPES pH7.5; 20% PEG3000