6M75

C-Myc DNA binding protein complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2019-09-16
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 21 21 2
Unit cell lengths	83.680, 114.946, 27.426
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.660 - 2.570
R-factor	0.2137
Rwork	0.211
R-free	0.23580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fxl
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	Auto-Rickshaw
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.650	2.610
High resolution limit [Å]	2.560	2.570
Rmerge	0.166	0.998
Rmeas	0.175	1.052
Rpim	0.055	0.328
Number of reflections	9032	428
<I/σ(I)>	11.9	2.4
Completeness [%]	99.7	99.5
Redundancy	9.7	10
CC(1/2)	0.995	0.711

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.07 M HEPES pH 7.5, 0.05 M Magnesium Sulfate, 1.8 M Lithium Sulfate