6M73

Crystal structure of Enterococcus hirae L-lactate oxidase in complex with D-lactate form ligand

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-10
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0
Spacegroup name	I 4 2 2
Unit cell lengths	137.960, 137.960, 127.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.530 - 1.700
R-factor	0.1326
Rwork	0.132
R-free	0.15430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4yl2
RMSD bond length	0.005
RMSD bond angle	1.278
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.530	19.530	1.740
High resolution limit [Å]	1.700	7.600	1.700
Rmerge	0.085	0.033	0.762
Rmeas	0.089	0.034	0.792
Total number of observations	889509
Number of reflections	67513	807	4953
<I/σ(I)>	22.2	59.41	4.3
Completeness [%]	99.9	92.9	100
Redundancy	13.175	12.462	13.705
CC(1/2)	0.999	0.999	0.930

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	Tris, PEG MME 2000