6M6W

Crystal structure the toxin-antitoxin MntA-HpeT mutant-Y104A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	54.102, 100.819, 131.781
Unit cell angles	90.00, 97.09, 90.00

Refinement procedure

Resolution	39.190 - 2.611
R-factor	0.2159
Rwork	0.214
R-free	0.24330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5yep
RMSD bond length	0.008
RMSD bond angle	1.105
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	EPMR
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	79.850	2.750
High resolution limit [Å]	2.610	2.610
Number of reflections	286711	41811
<I/σ(I)>	13.7
Completeness [%]	99.1
Redundancy	6.8
CC(1/2)	0.999	0.815

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.2	289.15	20%peg4000, 0.1 M HEPES,pH 7.5