6M6U

Crystal structure the toxin-antitoxin MntA-HpeT mutant-D39ED41E

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97982
Spacegroup name	P 1 21 1
Unit cell lengths	54.217, 99.954, 131.798
Unit cell angles	90.00, 96.11, 90.00

Refinement procedure

Resolution	53.908 - 2.349
R-factor	0.2051
Rwork	0.204
R-free	0.23270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5yep
RMSD bond length	0.004
RMSD bond angle	0.668
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.100	2.410
High resolution limit [Å]	2.349	2.349
Number of reflections	65138	4450
<I/σ(I)>	16.6
Completeness [%]	99.7
Redundancy	6.8
CC(1/2)	0.999	0.744

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	30%(v/v)Jeffamine M-600 PH7.0 100mM HEPES/Sodium hydroxide PH7.0