6M6Q

Crystal structure of Caenorhabditis elegans Dicer-related helicase 3 (DRH-3) N-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-15
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	0.9998
Spacegroup name	P 41 21 2
Unit cell lengths	93.180, 93.180, 215.958
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	70.640 - 2.800
R-factor	0.23632
Rwork	0.234
R-free	0.28597
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.188
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	Auto-Rickshaw
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	85.560	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.134	0.896
Rmeas	0.146	0.992
Number of reflections	24012	3401
<I/σ(I)>	10.9	2.2
Completeness [%]	99.3	99
Redundancy	11.1	10.2
CC(1/2)	0.996	0.752

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	277.15	0.2 M ammonium fluoride (pH 6.2), 20% w/v polyethylene glycol 3350, 5 mM dithiothreitol