6M5P
A class C beta-lactamase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-01 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 129.918, 60.233, 54.518 |
| Unit cell angles | 90.00, 110.71, 90.00 |
Refinement procedure
| Resolution | 34.461 - 1.250 |
| Rwork | 0.174 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6k8x |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.998 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.11) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.390 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Number of reflections | 102328 | 4615 |
| <I/σ(I)> | 31.55 | |
| Completeness [%] | 94.1 | |
| Redundancy | 5.4 | |
| CC(1/2) | 0.766 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 295 | 30% polyethylene glycol 1500, 0.1M sodium cacodylate (pH 5.5), 0.2M sodium chloride |
