6M5M
SPL-1 - GlcNAc complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-21 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 65 |
| Unit cell lengths | 44.460, 44.460, 303.613 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.230 - 1.700 |
| R-factor | 0.2037 |
| Rwork | 0.203 |
| R-free | 0.21610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6a7t |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.928 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.400 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.050 | 0.910 |
| Number of reflections | 37198 | 1950 |
| <I/σ(I)> | 30 | 3.3 |
| Completeness [%] | 100.0 | |
| Redundancy | 20.8 | |
| CC(1/2) | 1.000 | 0.942 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 10mM CaCl2, 10mM GlcNAc, 28% PEG 8000, 0.1M Tris-HCl (pH8.5), 0.2M sodium acetate, 10% glycerol |
