6M5A

Crystal structure of GH121 beta-L-arabinobiosidase HypBA2 from Bifidobacterium longum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-04
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.100
Spacegroup name	P 21 21 21
Unit cell lengths	81.526, 88.616, 127.918
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.350 - 1.850
R-factor	0.1594
Rwork	0.158
R-free	0.18550
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.633
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	Aimless
Refinement software	REFMAC (5.8.0253)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.350	1.890
High resolution limit [Å]	1.850	1.850
Rmerge	0.123	1.405
Rpim	0.051	0.571
Number of reflections	79741	4473
<I/σ(I)>	9.1	1.3
Completeness [%]	100.0	100
Redundancy	6.8	7
CC(1/2)	0.940	0.634

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.6	293	17% (w/v) PEG1000 and 0.1 M Tris-HCl (pH 6.6)