6M44
355 bp di-nucleosome harboring cohesive DNA termini (high cryoprotectant)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 98 |
| Detector technology | PIXEL |
| Collection date | 2019-08-26 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.038, 218.595, 115.931 |
| Unit cell angles | 90.00, 90.22, 90.00 |
Refinement procedure
| Resolution | 48.030 - 3.810 |
| R-factor | 0.2093 |
| Rwork | 0.208 |
| R-free | 0.28640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ut9 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.266 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.030 | 3.960 |
| High resolution limit [Å] | 3.810 | 3.810 |
| Rmerge | 0.960 | 1.454 |
| Rpim | 0.040 | 0.646 |
| Number of reflections | 41344 | 4614 |
| <I/σ(I)> | 11.4 | 1.2 |
| Completeness [%] | 99.8 | |
| Redundancy | 6.8 | |
| CC(1/2) | 0.999 | 0.424 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 291 | Calcium chloride, Potassium chloride, Potassium Cacodylate, Poly glutamic acid |
