6M40

Crystal structure of the NS3-like helicase from Alongshan virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	42.875, 58.674, 191.466
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.870 - 2.890
R-factor	0.2485
Rwork	0.246
R-free	0.29390
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	XDS
Phasing software	autoSHARP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.870	3.070
High resolution limit [Å]	2.890	2.890
Rmerge	0.179	1.430
Rmeas	0.193	1.550
Number of reflections	20593	3256
<I/σ(I)>	9.68	1.59
Completeness [%]	99.1	96.4
Redundancy	7.05	6.79
CC(1/2)	0.996	0.750

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIQUID DIFFUSION		293	0.16 M calcium acetate, 20% (v/v) PEG3350 and 5mM TECP