6M3U

Crystal structure of the mouse endonuclease EndoG(H138A/C100A), space group C2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	95
Detector technology	CCD
Collection date	2016-03-09
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	252.407, 81.340, 54.684
Unit cell angles	90.00, 102.17, 90.00

Refinement procedure

Resolution	42.190 - 2.320
R-factor	0.2032
Rwork	0.201
R-free	0.23750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6m3f
RMSD bond length	0.006
RMSD bond angle	1.155
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.190	2.370
High resolution limit [Å]	2.320	2.320
Number of reflections	45823	2429
<I/σ(I)>	16.94
Completeness [%]	98.1	95.8
Redundancy	10.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	24% PEG 3350, 0.1M HEPES at pH 7.5, 0.18M Ammonium sulfate