6M3F

Crystal structure of the mouse endonuclease EndoG(H138A/C110A), space group P212121

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 6D
Synchrotron site	PAL/PLS
Beamline	6D
Temperature [K]	193
Detector technology	CCD
Collection date	2015-02-09
Detector	ADSC QUANTUM 270
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	53.693, 68.880, 127.195
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.240 - 1.960
R-factor	0.1714
Rwork	0.169
R-free	0.21900
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	0.846
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.020
High resolution limit [Å]	1.960	1.960
Number of reflections	34168	2574
<I/σ(I)>	20.36
Completeness [%]	90.9
Redundancy	11.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	24% PEG 3350, 0.1M HEPES pH7.5, 0.18M Ammonium sulfate