6M36

The crystal structure of B. subtilis RsbV/RsbW complex in the monoclinic crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-09-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97933
Spacegroup name	P 1 21 1
Unit cell lengths	117.230, 70.940, 137.680
Unit cell angles	90.00, 105.35, 90.00

Refinement procedure

Resolution	29.788 - 3.400
R-factor	0.2115
Rwork	0.207
R-free	0.29030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1thn 1til
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (dev_3051)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.470	3.610
High resolution limit [Å]	3.400	3.400
Rmerge	0.147	0.492
Number of reflections	29346	2377
<I/σ(I)>	6.8	2.4
Completeness [%]	96.7
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	8.5	293	25% w/v PEG 1500 and 100 mM SPG buffer