6M2M

A role for histone chaperone OsChz1 in histone recognition and deposition

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-05-31
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97915
Spacegroup name	C 1 2 1
Unit cell lengths	167.361, 116.336, 101.904
Unit cell angles	90.00, 114.98, 90.00

Refinement procedure

Resolution	30.000 - 2.850
R-factor	0.1964
Rwork	0.194
R-free	0.25310
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	1.738
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.950
High resolution limit [Å]	2.850	6.120	2.850
Rmerge	0.098	0.033	0.465
Rmeas	0.117	0.038	0.567
Rpim	0.063	0.020	0.320
Total number of observations	134001
Number of reflections	41445	4267	4034
<I/σ(I)>	7.5
Completeness [%]	99.2	99.9	98.3
Redundancy	3.2	3.7	2.8
CC(1/2)		0.998	0.645

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	0.2M Sodium citrate,0.1M Tris-HCl pH 8.5, 30%(w/v)