6M2J

Uncommon structural features of rabbit MHC class I (RLA-A1) complexed with rabbit haemorrhagic disease virus (RHDV) derived peptide, VP60-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	CMOS
Collection date	2019-06-19
Detector	SDMS
Wavelength(s)	0.97911
Spacegroup name	P 21 21 21
Unit cell lengths	49.625, 74.361, 155.669
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.280 - 2.200
R-factor	0.2042
Rwork	0.202
R-free	0.25640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5deg
RMSD bond length	0.008
RMSD bond angle	0.963
Data reduction software	PROTEUM PLUS
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.174	0.955
Number of reflections	29903	29903
<I/σ(I)>	2.41
Completeness [%]	99.7
Redundancy	9.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	0.1M imidazole (pH 7.0), 12% w/v polyethylene glycol 2000